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plasmids pcdna3 1 flag his atm wild type wt  (Addgene inc)


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    Addgene inc plasmids pcdna3 1 flag his atm wild type wt
    (A) A549 <t>wild-type</t> and ATM KO A549 cells were treated with increasing concentrations of MnCL 2 . Total cell lysates were collected 24 hours post-treatment using RIPA buffer and analyzed by Western blot using antibodies against ATM, TBK1, and p-TBK1. β-actin served as a loading control. (B) A549 and ATM KO A549 cells were treated with the indicated concentrations of MnCL 2 , followed by stimulation with linearized DNA 24 hours later. After an additional 24 hours, total RNA was extracted, and IFN-λ1 mRNA expression levels were measured by quantitative RT-PCR and normalized to GAPDH. (C–F) 293T cells ( C ), HeLa cells ( D ), MDMs ( E ), and PHA-activated CD4⁺ T cells ( F ) were treated with varying doses of MnCL 2 . Whole cell lysates were collected after 24 hours and analyzed by Western blot using anti-ATM, anti-p-TBK1, and anti-TBK1 antibodies. β-actin was included as an internal loading control.
    Plasmids Pcdna3 1 Flag His Atm Wild Type Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmids+pcdna3+1+flag+his+atm+wild+type+wt/pcDNA3%2E1(%2B)Flag-His-ATM+wt+(Plasmid+%2331985)/bio_rxiv__2025__08__20__671272-217-1-12
    Average 93 stars, based on 71 article reviews
    plasmids pcdna3 1 flag his atm wild type wt - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "Manganese mediates antiviral effects by driving an ATM-TBK1 phosphorylation signaling pathway"

    Article Title: Manganese mediates antiviral effects by driving an ATM-TBK1 phosphorylation signaling pathway

    Journal: bioRxiv

    doi: 10.1101/2025.08.20.671272

    (A) A549 wild-type and ATM KO A549 cells were treated with increasing concentrations of MnCL 2 . Total cell lysates were collected 24 hours post-treatment using RIPA buffer and analyzed by Western blot using antibodies against ATM, TBK1, and p-TBK1. β-actin served as a loading control. (B) A549 and ATM KO A549 cells were treated with the indicated concentrations of MnCL 2 , followed by stimulation with linearized DNA 24 hours later. After an additional 24 hours, total RNA was extracted, and IFN-λ1 mRNA expression levels were measured by quantitative RT-PCR and normalized to GAPDH. (C–F) 293T cells ( C ), HeLa cells ( D ), MDMs ( E ), and PHA-activated CD4⁺ T cells ( F ) were treated with varying doses of MnCL 2 . Whole cell lysates were collected after 24 hours and analyzed by Western blot using anti-ATM, anti-p-TBK1, and anti-TBK1 antibodies. β-actin was included as an internal loading control.
    Figure Legend Snippet: (A) A549 wild-type and ATM KO A549 cells were treated with increasing concentrations of MnCL 2 . Total cell lysates were collected 24 hours post-treatment using RIPA buffer and analyzed by Western blot using antibodies against ATM, TBK1, and p-TBK1. β-actin served as a loading control. (B) A549 and ATM KO A549 cells were treated with the indicated concentrations of MnCL 2 , followed by stimulation with linearized DNA 24 hours later. After an additional 24 hours, total RNA was extracted, and IFN-λ1 mRNA expression levels were measured by quantitative RT-PCR and normalized to GAPDH. (C–F) 293T cells ( C ), HeLa cells ( D ), MDMs ( E ), and PHA-activated CD4⁺ T cells ( F ) were treated with varying doses of MnCL 2 . Whole cell lysates were collected after 24 hours and analyzed by Western blot using anti-ATM, anti-p-TBK1, and anti-TBK1 antibodies. β-actin was included as an internal loading control.

    Techniques Used: Western Blot, Control, Expressing, Quantitative RT-PCR

    ATM KO 293T cells were overexpressed by transfecting plasmids encoding wild type ATM (wt ATM), mutated ATM at kinase domain (ATM kd), mutated ATM at S1981A (ATM S1981A), respectively, and treated with Mn at varying concentrations. The total cell lysate was collected at 24 h after Mn treatment and subjected to western blot analysis using anti-ATM, p-TBK1, TBK1 antibodies, anti-β-actin was included as internal loading control. Untransfected 293T cells were also included in the experiment to serve as a positive control.
    Figure Legend Snippet: ATM KO 293T cells were overexpressed by transfecting plasmids encoding wild type ATM (wt ATM), mutated ATM at kinase domain (ATM kd), mutated ATM at S1981A (ATM S1981A), respectively, and treated with Mn at varying concentrations. The total cell lysate was collected at 24 h after Mn treatment and subjected to western blot analysis using anti-ATM, p-TBK1, TBK1 antibodies, anti-β-actin was included as internal loading control. Untransfected 293T cells were also included in the experiment to serve as a positive control.

    Techniques Used: Western Blot, Control, Positive Control

    Related Articles

    Mutagenesis:

    Article Title: Manganese mediates antiviral effects by driving an ATM-TBK1 phosphorylation signaling pathway
    Article Snippet: Isolated cells were stimulated with 5 μg/mL phytohemagglutinin (PHA; Sigma-Aldrich, St. Louis, MO, USA) for three days in RPMI-1640 medium (Thermo Fisher Scientific) supplemented with 10% FBS (Thermo Fisher Scientific), 25 mM HEPES (Quality Biological), and 5 μg/mL gentamicin (Thermo Fisher Scientific). .. The plasmids pcDNA3.1(+) Flag-His-ATM wild-type (wt), pcDNA3.1(+) Flag-His-ATM kinase-dead (kd), and hATM-S1981A (Addgene, Watertown, MA, USA) were used to overexpress either wild-type or mutant forms of ATM. .. The plasmid PEF1α-V5-His-TBK1, constructed from the empty vector pEF1α (Thermo Fisher Scientific), was used to overexpress TBK1 for co-immunoprecipitation assays.



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    plasmids pcdna3 1 flag his atm wild type wt  (Addgene inc)
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    Addgene inc plasmids pcdna3 1 flag his atm wild type wt
    (A) A549 <t>wild-type</t> and ATM KO A549 cells were treated with increasing concentrations of MnCL 2 . Total cell lysates were collected 24 hours post-treatment using RIPA buffer and analyzed by Western blot using antibodies against ATM, TBK1, and p-TBK1. β-actin served as a loading control. (B) A549 and ATM KO A549 cells were treated with the indicated concentrations of MnCL 2 , followed by stimulation with linearized DNA 24 hours later. After an additional 24 hours, total RNA was extracted, and IFN-λ1 mRNA expression levels were measured by quantitative RT-PCR and normalized to GAPDH. (C–F) 293T cells ( C ), HeLa cells ( D ), MDMs ( E ), and PHA-activated CD4⁺ T cells ( F ) were treated with varying doses of MnCL 2 . Whole cell lysates were collected after 24 hours and analyzed by Western blot using anti-ATM, anti-p-TBK1, and anti-TBK1 antibodies. β-actin was included as an internal loading control.
    Plasmids Pcdna3 1 Flag His Atm Wild Type Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmids+pcdna3+1+flag+his+atm+wild+type+wt/pcDNA3%2E1(%2B)Flag-His-ATM+wt+(Plasmid+%2331985)/bio_rxiv__2025__08__20__671272-217-1-12
    Average 93 stars, based on 1 article reviews
    plasmids pcdna3 1 flag his atm wild type wt - by Bioz Stars, 2026-09
    93/100 stars
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    Image Search Results


    (A) A549 wild-type and ATM KO A549 cells were treated with increasing concentrations of MnCL 2 . Total cell lysates were collected 24 hours post-treatment using RIPA buffer and analyzed by Western blot using antibodies against ATM, TBK1, and p-TBK1. β-actin served as a loading control. (B) A549 and ATM KO A549 cells were treated with the indicated concentrations of MnCL 2 , followed by stimulation with linearized DNA 24 hours later. After an additional 24 hours, total RNA was extracted, and IFN-λ1 mRNA expression levels were measured by quantitative RT-PCR and normalized to GAPDH. (C–F) 293T cells ( C ), HeLa cells ( D ), MDMs ( E ), and PHA-activated CD4⁺ T cells ( F ) were treated with varying doses of MnCL 2 . Whole cell lysates were collected after 24 hours and analyzed by Western blot using anti-ATM, anti-p-TBK1, and anti-TBK1 antibodies. β-actin was included as an internal loading control.

    Journal: bioRxiv

    Article Title: Manganese mediates antiviral effects by driving an ATM-TBK1 phosphorylation signaling pathway

    doi: 10.1101/2025.08.20.671272

    Figure Lengend Snippet: (A) A549 wild-type and ATM KO A549 cells were treated with increasing concentrations of MnCL 2 . Total cell lysates were collected 24 hours post-treatment using RIPA buffer and analyzed by Western blot using antibodies against ATM, TBK1, and p-TBK1. β-actin served as a loading control. (B) A549 and ATM KO A549 cells were treated with the indicated concentrations of MnCL 2 , followed by stimulation with linearized DNA 24 hours later. After an additional 24 hours, total RNA was extracted, and IFN-λ1 mRNA expression levels were measured by quantitative RT-PCR and normalized to GAPDH. (C–F) 293T cells ( C ), HeLa cells ( D ), MDMs ( E ), and PHA-activated CD4⁺ T cells ( F ) were treated with varying doses of MnCL 2 . Whole cell lysates were collected after 24 hours and analyzed by Western blot using anti-ATM, anti-p-TBK1, and anti-TBK1 antibodies. β-actin was included as an internal loading control.

    Article Snippet: The plasmids pcDNA3.1(+) Flag-His-ATM wild-type (wt), pcDNA3.1(+) Flag-His-ATM kinase-dead (kd), and hATM-S1981A (Addgene, Watertown, MA, USA) were used to overexpress either wild-type or mutant forms of ATM.

    Techniques: Western Blot, Control, Expressing, Quantitative RT-PCR

    ATM KO 293T cells were overexpressed by transfecting plasmids encoding wild type ATM (wt ATM), mutated ATM at kinase domain (ATM kd), mutated ATM at S1981A (ATM S1981A), respectively, and treated with Mn at varying concentrations. The total cell lysate was collected at 24 h after Mn treatment and subjected to western blot analysis using anti-ATM, p-TBK1, TBK1 antibodies, anti-β-actin was included as internal loading control. Untransfected 293T cells were also included in the experiment to serve as a positive control.

    Journal: bioRxiv

    Article Title: Manganese mediates antiviral effects by driving an ATM-TBK1 phosphorylation signaling pathway

    doi: 10.1101/2025.08.20.671272

    Figure Lengend Snippet: ATM KO 293T cells were overexpressed by transfecting plasmids encoding wild type ATM (wt ATM), mutated ATM at kinase domain (ATM kd), mutated ATM at S1981A (ATM S1981A), respectively, and treated with Mn at varying concentrations. The total cell lysate was collected at 24 h after Mn treatment and subjected to western blot analysis using anti-ATM, p-TBK1, TBK1 antibodies, anti-β-actin was included as internal loading control. Untransfected 293T cells were also included in the experiment to serve as a positive control.

    Article Snippet: The plasmids pcDNA3.1(+) Flag-His-ATM wild-type (wt), pcDNA3.1(+) Flag-His-ATM kinase-dead (kd), and hATM-S1981A (Addgene, Watertown, MA, USA) were used to overexpress either wild-type or mutant forms of ATM.

    Techniques: Western Blot, Control, Positive Control